Solution Information | help | |
Enzyme: | cGMP-specific 3',5'-cyclic phosphodiesterase | |
inhibitor: | BDBM23620 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.5 | |
Temp. Prep.: | 24 | |
Comments: | n/a | |
pH Buffer 1: | Tris-HCl | |
Concentration: | 20 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 2: | MgCl2 | |
Concentration: | 10 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 3: | DTT | |
Concentration: | 1 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
pH Buffer 4: | [3H]AMP or [3H]cGMP | |
Concentration: | 20,000 - 40,000 cpm/assay | |
ΔHion: | n/a | |
Comments: | n/a | |
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